AAi tool

Antibacterial Agent Index scoring and visualization

AAi assessment form

7 criteria · max 70

MIC and time–kill criteria refer to standard strains Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis under standardized conditions (Mueller–Hinton, 35–37 °C, aerobic; MIC incubation 16–24 h; time–kill sampling 0–24 h at 1×–4× MIC).

1. Antibacterial activity (MIC-based)

1a. MIC pattern (µg/mL) vs the four standard strains

1b. Comparison with standard drug (in vitro MIC)

Section max: 6 (MIC) + 4 (comparison) = 10 points.

2. Bacterial spectrum

Spectrum coverage

3. Mechanism of action

4. Time–kill kinetics

log₁₀ CFU/mL reduction across the four strains

5. Nanotechnology

Nanomaterials present (10 pts) vs absent (0 pts)

6. Green chemistry (precursors)

7. Cost

Cost (USD per gram)

< 5 USD/g → 10 pts; 5–50 USD/g → 6 pts; > 50 USD/g → 2 pts; enter 0 if unknown (0 pts).