AAi tool
AAi tool
Antibacterial Agent Index scoring and visualization
AAi assessment form
8 criteria
1. MIC value
Choose the compound type
Compounds with breakpoints
Compounds without breakpoints
What is the breakpoints
Susceptible (7 points)
Intermediate (5 points)
Resistant (2 points)
What is the MIC value (µg/mL)
≤ 5 (5 points)
5-10 (3 points)
> 10 (1 point)
Comparison with standard / What is the comparison outcome
Higher potency (2 points)
Same potency (1 point)
Less potency (0 points)
No comparison available (0 points)
2. Bacterial spectrum
Gram-positive + Gram-negative + resistant strains
Gram-positive + Gram-negative (no resistant strains reported)
Only Gram-positive or only Gram-negative
3. Mechanism of action
Novel/distinctive mechanisms overcoming resistance
Well-known mechanisms, still effective
Non-specific or linked to widespread resistance
4. Type of action
Bactericidal or bacteriostatic (reported)
Bactericidal only
Bacteriostatic only
5. Time–Kill Kinetics
≥3 log10 reduction within 6 hours
≥3 log10 reduction within 6–12 hours
≥3 log10 reduction within 12–24 hours
1–<3 log10 reduction within 12–24 hours
<1 log10 reduction within 12–24 hours or not studied
6. Integration of nanotechnology
Includes nanomaterials (Nanozymes, NPs, CQDs, MOFs, nanocomposites)
7. Cost of the compound
Estimated cost (USD)
≤ 5 → 3 points, ≤ 50 → 2 points, ≤ 100 → 1 point
8. Green precursors
Natural resources only
Natural resources with chemical inputs
Chemical sources only
Calculate AAi Score
