About AAi Tool

Antibacterial Assessment Index (AAi): An Open-Access Software for Comprehensive Antibacterial Agent Evaluation

Authors

  • Rehab H. Elattara,b
  • Reem H. Obaydo*c
  • Samir Khanjid
  • Samar H. Elagamyb

Affiliations

  • a Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
  • b Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt
  • c Analytical and Food Chemistry Department, Faculty of Pharmacy, Ebla Private University, Idlib, Syria
  • d Informatics and Communications Department, Faculty of Engineering, Ebla Private University, Idlib, Syria

Corresponding Author

Obaydo.reem@gmail.com

Authors Email

All rights reserved to the authors. Version 1.0, 2026.

How the AAi Tool Works

The AAi tool page is an ASP.NET Core Razor Page. When you first visit, the form loads with default values for seven criteria. When you click "Calculate AAi Score", the form is submitted (POST), and the server calculates a score based on your answers.

The 7 Criteria and Scoring (max 70)

Scores follow the published AAi rubric (MIC and time–kill refer to E. coli, P. aeruginosa, S. aureus, and E. faecalis under standardized Mueller–Hinton conditions):

  • 1. Antibacterial activity (MIC-based) (max 10): MIC pattern across four strains (0–6) plus in vitro comparison with a standard drug (0–4).
  • 2. Bacterial spectrum (max 10): Gram-positive, Gram-negative, and resistant (10); Gram-positive and Gram-negative (6); one category only (2).
  • 3. Mechanism of action (max 10): Novel or resistance-overcoming (10); well-known but still effective (6); non-specific or resistance-linked (2).
  • 4. Time–kill kinetics (max 10): Eleven graded outcomes from full rapid kill across strains (10) to no ≥3 log₁₀ reduction within 24 h (0).
  • 5. Nanotechnology (max 10): Nanomaterials present (10) or absent (0).
  • 6. Green chemistry (precursors) (max 10): Natural resources (10); natural and chemical (6); chemicals only (2).
  • 7. Cost (max 10): USD per gram — below 5 (10), 5–50 (6), above 50 (2); unknown cost entered as 0 (0 points).

Classification

  • 50–70 → Excellent antibacterial agent
  • 25–49 → Moderate antibacterial agent
  • < 25 → Weak antibacterial agent

The result is displayed with a circular gauge (percentage of 70) and can be exported as a PNG image.