About AAi Tool

Antibacterial Assessment Index (AAi): An Open-Access Software for Comprehensive Antibacterial Agent Evaluation

Authors

  • Rehab H. Elattara,b
  • Reem H. Obaydo*c
  • Samir Khanjid
  • Samar H. Elagamyb

Affiliations

  • a Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
  • b Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt
  • c Analytical and Food Chemistry Department, Faculty of Pharmacy, Ebla Private University, Idlib, Syria
  • d Informatics and Communications Department, Faculty of Engineering, Ebla Private University, Idlib, Syria

Corresponding Author

Obaydo.reem@gmail.com

Authors Email

All rights reserved to the authors. Version 1.0, 2026.

How the AAi Tool Works

The AAi tool page is an ASP.NET Core Razor Page. When you first visit, the form loads with default values for six criteria (Table 1 in the AAI point-system document). When you click "Calculate AAi Score", the form is submitted (POST), and the server calculates a score based on your answers.

The 6 Criteria and Scoring (base max 50)

Scores follow the AAI document (MIC and time–kill use E. coli, P. aeruginosa, S. aureus, and E. faecalis):

  • 1. Antibacterial activity (MIC-based) (max 10): MIC value pattern (0–6) plus comparison with standard drug** (0–4).
  • 2. Bacterial spectrum (max 10): Gram+, Gram−, and resistant (10); Gram+ and Gram− (6); Gram-positive or Gram-negative only (2).
  • 3. Mechanism of action (max 10): Three mechanism tiers (10 / 6 / 2) as in the table.
  • 4. Time–kill kinetics*** (max 10): log₁₀ CFU/mL pattern (0–6) plus time frame — average time for all strains (0–4).
  • 5. Green chemistry (max 10): Natural precursors (10); natural with chemical resources (6); chemicals (2).
  • 6. Additional considerations: Nano technology integration — Yes (0) / No (−2); Cost — 5–100 (0) / >100 (−2). These adjust the total downward from the first five sections only.

Classification

Bands are scaled from the prior 70-point cutoffs (50 and 25) to a 50-point total:

  • 36–50 → Excellent antibacterial agent
  • 18–35 → Moderate antibacterial agent
  • < 18 → Weak antibacterial agent

The result is displayed with a circular gauge (percentage of 50) and can be exported as a PNG image.